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Stem Cell Processing After the stem cells are collected by the process of apheresis, they are taken to the Stem Cell Processing Unit where they will undergo the following procedures: 1. Stem Cell Enumeration: The cells will be counted using a machine call a flow cytometer. This is a very sophisticated device that will specifically count the stem cells using a laser light.
2. Cell Viability Studies: A portion of the stem cell product will be placed in a cell culture system to verify that the cells which were collected are indeed healthy cells.
3. Infection Testing: A portion of the stem cell product will be placed in a bacterial culture system to ensure that the cells have not been infected during the collection and processing procedures.
4. Cryopreservation: The final stem cell product will have a preservative added (DMSO) to ensure that the cells freeze safely. The stem cells will then be placed in a controlled rate freezer to lower the temperature of the cells at a specific rate.
5. Storage: Finally, the stem cells are stored in liquid nitrogen.
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